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ScienCellGK094Human Mesenchymal Stem Cell Differentiation qPCR ArrayKit
更新時間:2023-01-03   點擊次數(shù):527次

GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK094

產(chǎn)品名稱:GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK094 ScienCell Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人間充質干細胞分化 qPCR 陣列試劑盒 (GQH-MSC) 旨在促進與間充質干細胞 (MSC) 生物學、多能性維持和分化相關的 88 個關鍵基因的基因表達譜分析。下面顯示了如何對所包含的基因進行分組的簡要示例:  

MSC 標記:GNL3、BMPR1A、BMPR1B、BMPR2、KIT、NT5E、ENG、ALCAM、ANPEP、CDH2CD44、PTPRC、THY1HLA-A

干性標記:FUT4、PROM1、ABCG2、SOX2、FGF2INS、LIF

脂肪生成標志物:TNFRSF9ADIPOQ,ADIPOR1 / 2,CIDEA,CLTCL1DLK2,FABP4,SLC27As,SLC2A4,LEPPLIN2

軟骨生成標志物:ACAN,ANXA6,CD151,CHAD,FOXC1/2MATN1/3/4,MIA,OTOR,SOX5/6/9

肌源性前體標志物:CDH15,CAV1CD34FOXK1,MYF5,MYOD1MYOG,NCAM1,PAX3/7/9

骨祖標志物:ALPPDCN,MCAMMEPE,SP7RUNX2,THPO

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Differentiation qPCR Array Kit (GQH-MSC) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) biology, pluripotency maintenance, and differentiation. Brief examples of how included genes may be grouped are shown below:    

·         MSC markers: GNL3, BMPR1A, BMPR1B, BMPR2, KIT, NT5E, ENG, ALCAM, ANPEP, CDH2, CD44, PTPRC, THY1, HLA-A

·         Stemness markers: FUT4, PROM1, ABCG2, SOX2, FGF2, INS, LIF

·         Adipogenesis markers: TNFRSF9, ADIPOQ, ADIPOR1/2, CIDEA, CLTCL1, DLK2, FABP4, SLC27As, SLC2A4, LEP, PLIN2

·         Chondrogenesis markers: ACAN, ANXA6, CD151, CHAD, FOXC1/2, MATN1/3/4, MIA, OTOR, SOX5/6/9

·         Myogenic precursor markers: CDH15, CAV1, CD34, FOXK1, MYF5, MYOD1, MYOG, NCAM1, PAX3/7/9

·         Osteoprogenitor markers: ALPP, DCN, MCAM, MEPE, SP7, RUNX2, THPO

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人間充質干細胞分化qPCR陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ABCG2

ANXA6

CD44

DCN

FOXC2

ITGA1

MATN4

NGFR

PPARG

SLC27A6

TFRC

β-actin

B

ACAN

BMPR1A

CDH15

DLK1

FOXK1

ITGB1

MCAM

NT5E

PPARGC1A

SLC2A4

THPO

GAPDH

C

ADIPOQ

BMPR1B

CDH2

DLK2

FUT4

ITM2A

MEPE

OTOR

PROM1

SOX2

THY1

LDHA

D

ADIPOR1

BMPR2

CHAD

ENG

GNL3

KIT

MIA

PAX3

PTPRC

SOX5

TNFRSF9

NONO

E

ADIPOR2

BSCL2

CIDEA

FABP4

HLA-A

LEP

MYF5

PAX7

RUNX2

SOX6

UCP1

PPIH

F

ALCAM

CAV1

CLTCL1

FAM20B

IBSP

LIF

MYOD1

PAX9

SLC27A1

SOX9

VCAM1

GDC

G

ALPP

CD151

COL4A1

FGF2

ICAM1

MATN1

MYOG

PDGFRA

SLC27A2

SP7

VIM

PPC

H

ANPEP

CD34

CRTAC1

FOXC1

INS

MATN3

NCAM1

PLIN2

SLC27A4

SUSD2

ZIC1

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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